› 5jj2

X-ray diffraction
1.25Å resolution

Crystal structure of the central domain of human AKAP18 gamma/delta in complex with malonate

Released:
DOI: 10.2210/pdb5jj2/pdb
PDB entry 5jj2 coloured by chain, front view.
PDB entry 5jj2 coloured by chain, side view.
PDB entry 5jj2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Malonate in the nucleotide-binding site traps human AKAP18γ/δ in a novel conformational state.
Bjerregaard-Andersen K, Østensen E, Scott JD, Taskén K, Morth JP
Acta Crystallogr F Struct Biol Commun 72 591-7 (2016)
PMID: 27487922

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192802 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
A-kinase anchor protein 7 isoform gamma Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9P0M2 (Residues: 76-286; Coverage: 61%)
Gene names: AKAP18, AKAP7
Sequence domains: AKAP7 2'5' RNA ligase-like domain
Structure domains: Cyclic phosphodiesterase

Ligands and Environments

1 bound ligand:
Ligand MLI 6 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P212121
Unit cell:
a: 56.25Å b: 60.26Å c: 65.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Scaffold proteins as dynamic integrators of biological processes.
DiRusso et al. (2022)
4 other articles

1 mention without citation

Malonate in the nucleotide-binding site traps human AKAP18γ/δ in a novel conformational state.
Bjerregaard-Andersen et al. (2016)