› 5jj5

X-ray diffraction
2.3Å resolution

Crystal structure of iron uptake ABC transporter substrate-binding protein PiaA from Streptococcus pneumoniae Canada MDR_19A bound to hydroxymate siderophore ferrioxamine E and iron(III)

Released:
DOI: 10.2210/pdb5jj5/pdb
Model geometry
Fit model/data
PDB entry 5jj5 coloured by chain, front view.
PDB entry 5jj5 coloured by chain, side view.
PDB entry 5jj5 coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Entry authors: Stogios PJ, Wawrzak Z, Kurdritska M, Yim V, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 308 amino acids
Theoretical weight: 33.83 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A062WLD9 (Residues: 35-341; Coverage: 96%)
Gene names: AZJ70_08635, ERS019261_01558, ERS019486_01639, GM535_02610, GM536_10160, GM537_02370, GM539_03030, GM543_02280, GM545_06190, NCTC13734_01136, SAMEA2627268_02033, SAMEA2696453_01832, SAMEA2783718_01854, SAMEA3354366_02049, SAMEA3389245_02338, SAMEA3431391_00916, SAMEA4038883_01554, yhfQ
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

4 bound ligands:
Ligand FE 2 x FE
Ligand 6L0 2 x 6L0
Ligand CL 1 x CL
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 77.809Å b: 73.844Å c: 87.161Å
α: 90° β: 115.79° γ: 90°
R-values:
R R work R free
0.148 0.146 0.196
Expression system: Escherichia coli BL21(DE3)

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