› 5jlc

X-ray diffraction
2.4Å resolution

Function and Biology Details

Reaction catalysed: 
(1a) a 14-alpha-methylsteroid + [reduced NADPH--hemoprotein reductase] + O(2) = a 14-alpha-hydroxysteroid + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lanosterol 14-alpha demethylase Chain: A
Molecule details ›
Chain: A
Length: 515 amino acids
Theoretical weight: 59.33 KDa
Source organism: [Candida] glabrata CBS 138
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P50859 (Residues: 20-533; Coverage: 96%)
Gene names: CAGL0E04334g, CYP51, ERG11
Sequence domains: Cytochrome P450

Ligands and Environments

5 bound ligands:
Ligand HEM 1 x HEM
Ligand 1YN 1 x 1YN
Ligand PGE 1 x PGE
Ligand 1PE 1 x 1PE
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: I432
Unit cell:
a: 237.94Å b: 237.94Å c: 237.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.231
Expression system: Saccharomyces cerevisiae

Quick links

Citations

7 review citations

The Fungal CYP51s: Their Functions, Structures, Related Drug Resistance, and Inhibitors.
Zhang et al. (2019)
6 more

17 mentions without citation

Roles for Structural Biology in the Discovery of Drugs and Agrochemicals Targeting Sterol 14α-Demethylases.
Monk et al. (2021)
16 more