› 5jmf

X-ray diffraction
1.85Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heparin-sulfate lyase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 655 amino acids
Theoretical weight: 77.09 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q89YS4 (Residues: 23-666; Coverage: 97%)
Gene name: BT_4657
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand TRS 1 x TRS
Ligand MG 1 x MG
1 modified residue:
Ligand MLY 25 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2
Unit cell:
a: 186.69Å b: 45.741Å c: 80.514Å
α: 90° β: 94.05° γ: 90°
R-values:
R R work R free
0.166 0.165 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Marine Polysaccharides: Occurrence, Enzymatic Degradation and Utilization.
Bäumgen et al. (2021)
8 other articles

2 mentions without citation

Conformational flexibility of PL12 family heparinases: structure and substrate specificity of heparinase III from Bacteroides thetaiotaomicron (BT4657).
Ulaganathan et al. (2017)
1 more