› 5jo7

X-ray diffraction
2.15Å resolution

Henbane premnaspirodiene synthase (HPS), also known as Henbane vetispiradiene synthase (HVS) from Hyoscyamus muticus

Released:
DOI: 10.2210/pdb5jo7/pdb
Model geometry
Fit model/data
PDB entry 5jo7 coloured by chain, front view.
PDB entry 5jo7 coloured by chain, side view.
PDB entry 5jo7 coloured by chain, top view.
Source organism: Hyoscyamus muticus
Entry authors: Koo HJ, Xu Y, Louie GV, Bowman M, Noel JP

Function and Biology Details

Reaction catalysed: 
(2E,6E)-farnesyl diphosphate = vetispiradiene + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174467 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vetispiradiene synthase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 538 amino acids
Theoretical weight: 62.52 KDa
Source organism: Hyoscyamus muticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q39978 (Residues: 23-555; Coverage: 96%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 100.449Å b: 100.69Å c: 222.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.25
Expression system: Escherichia coli BL21(DE3)

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