› 5jom

X-ray diffraction
1.9Å resolution

X-ray structure of CO-bound sperm whale myoglobin using a fixed target crystallography chip

Released:
DOI: 10.2210/pdb5jom/pdb
PDB entry 5jom coloured by chain, front view.
PDB entry 5jom coloured by chain, side view.
PDB entry 5jom coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
Fixed target combined with spectral mapping: approaching 100% hit rates for serial crystallography.
Oghbaey S, Sarracini A, Ginn HM, Pare-Labrosse O, Kuo A, Marx A, Epp SW, Sherrell DA, Eger BT, Zhong Y, Loch R, Mariani V, Alonso-Mori R, Nelson S, Lemke HT, Owen RL, Pearson AR, Stuart DI, Ernst OP, Mueller-Werkmeister HM, Miller RJ
Acta Crystallogr D Struct Biol 72 944-55 (2016)
PMID: 27487825

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.37 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli
UniProt:
  • Canonical: P02185 (Residues: 1-154; Coverage: 100%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand HEM 1 x HEM
Ligand SO4 1 x SO4
Ligand CMO 1 x CMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE XPP
Spacegroup: P212121
Unit cell:
a: 38.13Å b: 46.483Å c: 84.521Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.259
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Sample Delivery Media for Serial Crystallography.
Nam KH. (2019)
1 more

3 mentions without citation

Fixed target combined with spectral mapping: approaching 100% hit rates for serial crystallography.
Oghbaey et al. (2016)
2 more