› 5joo

X-ray diffraction
1.41Å resolution

XFEL structure of influenza A M2 wild type TM domain at low pH in the lipidic cubic phase at room temperature

Released:
DOI: 10.2210/pdb5joo/pdb
PDB entry 5joo coloured by chain, front view.
PDB entry 5joo coloured by chain, side view.
PDB entry 5joo coloured by chain, top view.
Source organism: Influenza A virus (A/Hickox/1940(H1N1))
Primary publication:
XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction.
Thomaston JL, Woldeyes RA, Nakane T, Yamashita A, Tanaka T, Koiwai K, Brewster AS, Barad BA, Chen Y, Lemmin T, Uervirojnangkoorn M, Arima T, Kobayashi J, Masuda T, Suzuki M, Sugahara M, Sauter NK, Tanaka R, Nureki O, Tono K, Joti Y, Nango E, Iwata S, Yumoto F, Fraser JS, DeGrado WF
Proc Natl Acad Sci U S A 114 13357-13362 (2017)
PMID: 28835537

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Matrix protein 2 Chain: A
Molecule details ›
Chain: A
Length: 27 amino acids
Theoretical weight: 2.75 KDa
Source organism: Influenza A virus (A/Hickox/1940(H1N1))
Expression system: Not provided
UniProt:
  • Canonical: Q0HD59 (Residues: 22-46; Coverage: 26%)
Gene names: M, M2

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SACLA BEAMLINE BL3
Spacegroup: I4
Unit cell:
a: 30.051Å b: 30.051Å c: 67.399Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.237
Expression system: Not provided

Quick links

Citations

6 review citations

Influenza A Virus M2 Protein: Roles from Ingress to Egress.
Manzoor et al. (2017)
5 more

6 mentions without citation

Put a cork in it: Plugging the M2 viral ion channel to sink influenza.
Jalily et al. (2020)
5 more