› 5jre

X-ray diffraction
2.1Å resolution

Crystal structure of NeC3PO in complex with ssDNA.

Released:
DOI: 10.2210/pdb5jre/pdb
PDB entry 5jre coloured by chain, front view.
PDB entry 5jre coloured by chain, side view.
PDB entry 5jre coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for single-stranded RNA recognition and cleavage by C3PO.
Zhang J, Liu H, Yao Q, Yu X, Chen Y, Cui R, Wu B, Zheng L, Zuo J, Huang Z, Ma J, Gan J
Nucleic Acids Res 44 9494-9504 (2016)
PMID: 27596600

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118877 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Haloacid dehalogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 219 amino acids
Theoretical weight: 25.61 KDa
Source organism: Nanoarchaeum equitans Kin4-M
Expression system: Escherichia coli
UniProt:
  • Canonical: Q74ML9 (Residues: 1-185; Coverage: 100%)
Gene name: NEQ131
Sequence domains: Translin family
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
ssDNA Chains: I, J
Molecule details ›
Chains: I, J
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: unidentified
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ADE 5 x ADE
Ligand ADZ 3 x ADZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 98.83Å b: 128.2Å c: 102.18Å
α: 90° β: 95.22° γ: 90°
R-values:
R R work R free
0.218 0.216 0.259
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Translin: A multifunctional protein involved in nucleic acid metabolism.
Gupta et al. (2019)
6 other articles