› 5ju9

X-ray diffraction
1.18Å resolution

Structure of a beta-1,4-mannanase, SsGH134, in complex with Man3.

Released:
DOI: 10.2210/pdb5ju9/pdb
PDB entry 5ju9 coloured by chain, front view.
PDB entry 5ju9 coloured by chain, side view.
PDB entry 5ju9 coloured by chain, top view.
Source organism: Streptomyces sp. NRRL B-24361
Primary publication:
A β-Mannanase with a Lysozyme-like Fold and a Novel Molecular Catalytic Mechanism.
Jin Y, Petricevic M, John A, Raich L, Jenkins H, Portela De Souza L, Cuskin F, Gilbert HJ, Rovira C, Goddard-Borger ED, Williams SJ, Davies GJ
ACS Cent Sci 2 896-903 (2016)
PMID: 28058278

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-1,4-mannanase Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 17.59 KDa
Source organism: Streptomyces sp. NRRL B-24361
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A1L1QK13 (Residues: 1-168; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 134

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, BMA
Carbohydrate polymer
1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 36.68Å b: 53.63Å c: 82.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.132 0.131 0.153
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Enzymatic Transition States and Drug Design.
Schramm VL. (2018)
2 more

3 mentions without citation

A β-Mannanase with a Lysozyme-like Fold and a Novel Molecular Catalytic Mechanism.
Jin et al. (2016)
2 more