› 5jub

X-ray diffraction
2.57Å resolution

Crystal structure of ComR from S.thermophilus in complex with DNA and its signalling peptide ComS.

Released:
DOI: 10.2210/pdb5jub/pdb
PDB entry 5jub coloured by chain, front view.
PDB entry 5jub coloured by chain, side view.
PDB entry 5jub coloured by chain, top view.
Source organism: Streptococcus thermophilus LMD-9
Primary publication:
Structural Insights into Streptococcal Competence Regulation by the Cell-to-Cell Communication System ComRS.
Talagas A, Fontaine L, Ledesma-García L, Mignolet J, Li de la Sierra-Gallay I, Lazar N, Aumont-Nicaise M, Federle MJ, Prehna G, Hols P, Nessler S
PLoS Pathog 12 e1005980 (2016)
PMID: 27907189

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103529 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 310 amino acids
Theoretical weight: 36.55 KDa
Source organism: Streptococcus thermophilus LMD-9
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1L1QK15 (Residues: 1-310; Coverage: 100%)
Gene name: BN551_00358
Sequence domains:
ComS Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 amino acids
Theoretical weight: 883 Da
Source organism: Streptococcus thermophilus LMD-9
Expression system: Not provided
pComX-rev Chain: X
Molecule details ›
Chain: X
Length: 20 nucleotides
Theoretical weight: 6.14 KDa
pComX-for Chain: Z
Molecule details ›
Chain: Z
Length: 20 nucleotides
Theoretical weight: 6.12 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 186.11Å b: 46.19Å c: 100.27Å
α: 90° β: 98.23° γ: 90°
R-values:
R R work R free
0.229 0.227 0.268
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

Quorum Sensing Regulation of Competence and Bacteriocins in Streptococcus pneumoniae and mutans.
Shanker et al. (2017)
7 more

5 mentions without citation

Structural Insights into Streptococcal Competence Regulation by the Cell-to-Cell Communication System ComRS.
Talagas et al. (2016)
4 more