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› 5jud

X-ray diffraction
2.59Å resolution

Crystal structure of glucosyl-3-phosphoglycerate synthase from Mycobacterium tuberculosis in complex with uridine-diphosphate (UDP) - GpgS*UDP

Released:
DOI: 10.2210/pdb5jud/pdb
Model geometry
Fit model/data
PDB entry 5jud coloured by chain, front view.
PDB entry 5jud coloured by chain, side view.
PDB entry 5jud coloured by chain, top view.
Source organism: Mycobacterium tuberculosis variant bovis AF2122/97
Primary publication:
Structural Snapshots and Loop Dynamics along the Catalytic Cycle of Glycosyltransferase GpgS.
Albesa-Jové D, Romero-García J, Sancho-Vaello E, Contreras FX, Rodrigo-Unzueta A, Comino N, Carreras-González A, Arrasate P, Urresti S, Biarnés X, Planas A, Guerin ME
Structure 25 1034-1044.e3 (2017)
PMID: 28625787

Function and Biology Details

Reaction catalysed: 
an NDP-alpha-D-glucose + (2R)-3-phosphoglycerate = (2R)-2-O-(alpha-D-glucopyranosyl)-3-phospho-glycerate + a ribonucleoside 5'-diphosphate +H(+).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182033 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosyl-3-phosphoglycerate synthase Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 34.68 KDa
Source organism: Mycobacterium tuberculosis variant bovis AF2122/97
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7U0E1 (Residues: 1-324; Coverage: 100%)
Gene names: BQ2027_MB1240, gpgS
Sequence domains: Glycosyl transferase family 2
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

1 bound ligand:
Ligand UDP 1 x UDP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: I41
Unit cell:
a: 100.24Å b: 100.24Å c: 125.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.216
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Mycobacterium tuberculosis capsule: a cell structure with key implications in pathogenesis.
Kalscheuer et al. (2019)
1 more