› 5jvp

X-ray diffraction
2.1Å resolution

The neck-linker and alpha 7 helix of Homo sapiens CENP-E

Released:
DOI: 10.2210/pdb5jvp/pdb
PDB entry 5jvp coloured by chain, front view.
PDB entry 5jvp coloured by chain, side view.
PDB entry 5jvp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.
Phillips RK, Peter LG, Gilbert SP, Rayment I
J Biol Chem 291 20372-86 (2016)
PMID: 27462072

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Centromere-associated protein E; Microtubule-associated protein RP/EB family member 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 90 amino acids
Theoretical weight: 10.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02224 (Residues: 336-376; Coverage: 2%)
  • Canonical: Q15691 (Residues: 211-257; Coverage: 18%)
Gene names: CENPE, MAPRE1
Sequence domains: EB1-like C-terminal motif

Ligands and Environments

1 bound ligand:
Ligand CD 3 x CD
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 100.609Å b: 100.609Å c: 107.627Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.237 0.274
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Kinesin-2 motors: Kinetics and biophysics.
Gilbert et al. (2018)
2 more

2 mentions without citation

Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.
Phillips et al. (2016)
1 more