› 5jwa

X-ray diffraction
2.16Å resolution

Function and Biology Details

Reaction catalysed: 
NADH + a quinone = NAD(+) + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184652 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:ubiquinone reductase (non-electrogenic) Chains: A, H
Molecule details ›
Chains: A, H
Length: 521 amino acids
Theoretical weight: 60.3 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q8I302 (Residues: 25-533; Coverage: 96%)
Gene name: PF3D7_0915000
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
3 bound ligands:
Ligand MG 8 x MG
Ligand TRT 6 x TRT
Ligand ACT 12 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: I41
Unit cell:
a: 191.67Å b: 191.67Å c: 91.388Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.228
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

Quick links

Citations

1 review citation

Research journey of respirasome.
Wu et al. (2020)
14 other articles

5 mentions without citation

Structure of the NDH-2 - HQNO inhibited complex provides molecular insight into quinone-binding site inhibitors.
Petri et al. (2018)
4 more