› 5jwr

X-ray diffraction
2.61Å resolution

Crystal structure of foldswitch-stabilized KaiB in complex with the N-terminal CI domain of KaiC and a dimer of KaiA C-terminal domains from Thermosynechococcus elongatus

Released:
DOI: 10.2210/pdb5jwr/pdb
PDB entry 5jwr coloured by chain, front view.
PDB entry 5jwr coloured by chain, side view.
PDB entry 5jwr coloured by chain, top view.
Source organism: Thermosynechococcus vestitus BP-1
Primary publication:
Structural basis of the day-night transition in a bacterial circadian clock.
Tseng R, Goularte NF, Chavan A, Luu J, Cohen SE, Chang YG, Heisler J, Li S, Michael AK, Tripathi S, Golden SS, LiWang A, Partch CL
Science 355 1174-1180 (2017)
PMID: 28302851

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-181289 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Circadian clock oscillator protein KaiC Chains: A, C
Molecule details ›
Chains: A, C
Length: 247 amino acids
Theoretical weight: 27.74 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79V60 (Residues: 17-247; Coverage: 45%)
Gene names: kaiC, tlr0483
Sequence domains: KaiC
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Circadian clock oscillator protein KaiB Chains: B, D
Molecule details ›
Chains: B, D
Length: 99 amino acids
Theoretical weight: 10.94 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79V61 (Residues: 1-99; Coverage: 92%)
Gene names: kaiB, tlr0482
Sequence domains: KaiB domain
Structure domains: Glutaredoxin
Circadian clock oscillator protein KaiA Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 145 amino acids
Theoretical weight: 17.33 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79V62 (Residues: 147-283; Coverage: 48%)
Gene names: kaiA, tlr0481
Sequence domains: KaiA C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 74.595Å b: 79.105Å c: 80.838Å
α: 107.27° β: 90.71° γ: 111.2°
R-values:
R R work R free
0.188 0.185 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Gut microbiota as a transducer of dietary cues to regulate host circadian rhythms and metabolism.
Choi et al. (2021)
13 more

7 mentions without citation

Structure, function, and mechanism of the core circadian clock in cyanobacteria.
Swan et al. (2018)
6 more