› 5jxq

X-ray diffraction
1.2Å resolution

TRNA-GUANINE TRANSGLYCOSYLASE (TGT) IN COMPLEX WITH 6-AMINO-2-{[4-(2-HYDROXYETHYL)PHENETHYL]AMINO}-1,7-DIHYDRO-8H-IMIDAZO[4,5-g]QUINAZOLIN-8-ONE

Released:
DOI: 10.2210/pdb5jxq/pdb
PDB entry 5jxq coloured by chain, front view.
PDB entry 5jxq coloured by chain, side view.
PDB entry 5jxq coloured by chain, top view.
Source organism: Zymomonas mobilis subsp. mobilis
Primary publication:
Occupying a flat subpocket in a tRNA-modifying enzyme with ordered or disordered side chains: Favorable or unfavorable for binding?
Neeb M, Hohn C, Ehrmann FR, Härtsch A, Heine A, Diederich F, Klebe G
Bioorg Med Chem 24 4900-4910 (2016)
PMID: 27501913

Function and Biology Details

Reaction catalysed: 
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuine tRNA-ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 42.93 KDa
Source organism: Zymomonas mobilis subsp. mobilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P28720 (Residues: 1-386; Coverage: 100%)
Gene names: ZMO0363, tgt
Sequence domains: Queuine tRNA-ribosyltransferase
Structure domains: Queuine tRNA-ribosyltransferase-like

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand GOL 3 x GOL
Ligand 6OK 1 x 6OK
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C2
Unit cell:
a: 90.22Å b: 64.76Å c: 70.34Å
α: 90° β: 96.16° γ: 90°
R-values:
R R work R free
0.138 0.137 0.157
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Application of ITC-Based Characterization of Thermodynamic and Kinetic Association of Ligands With Proteins in Drug Design.
Su et al. (2018)
3 more