› 5jxz

X-ray diffraction
1.88Å resolution

A low magnesium structure of the isochorismate synthase, EntC

Released:
DOI: 10.2210/pdb5jxz/pdb
PDB entry 5jxz coloured by chain, front view.
PDB entry 5jxz coloured by chain, side view.
PDB entry 5jxz coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
An Open and Shut Case: The Interaction of Magnesium with MST Enzymes.
Meneely KM, Sundlov JA, Gulick AM, Moran GR, Lamb AL
J Am Chem Soc 138 9277-93 (2016)
PMID: 27373320

Function and Biology Details

Reaction catalysed: 
Chorismate = isochorismate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isochorismate synthase EntC Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 42.98 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEJ3 (Residues: 1-391; Coverage: 100%)
Gene names: ECs0632, Z0735, entC
Sequence domains: chorismate binding enzyme
Structure domains: Anthranilate synthase

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ISC 2 x ISC
Ligand ISJ 2 x ISJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P41212
Unit cell:
a: 80.205Å b: 80.205Å c: 272.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Nonribosomal peptides for iron acquisition: pyochelin biosynthesis as a case study.
Ronnebaum et al. (2018)
1 more

2 mentions without citation

Unraveling the Structure and Mechanism of the MST(ery) Enzymes.
Shelton et al. (2018)
1 more