› 5jza

X-ray diffraction
2.14Å resolution

Aspartyl/Asparaginyl beta-hydroxylase (AspH)oxygenase and TPR domains in complex with manganese and N-oxalylglycine

Released:
DOI: 10.2210/pdb5jza/pdb
PDB entry 5jza coloured by chain, front view.
PDB entry 5jza coloured by chain, side view.
PDB entry 5jza coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Aspartate/asparagine-β-hydroxylase crystal structures reveal an unexpected epidermal growth factor-like domain substrate disulfide pattern.
Pfeffer I, Brewitz L, Krojer T, Jensen SA, Kochan GT, Kershaw NJ, Hewitson KS, McNeill LA, Kramer H, Münzel M, Hopkinson RJ, Oppermann U, Handford PA, McDonough MA, Schofield CJ
Nat Commun 10 4910 (2019)
PMID: 31659163

Function and Biology Details

Reaction catalysed: 
Peptide L-aspartate + 2-oxoglutarate + O(2) = peptide 3-hydroxy-L-aspartate + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartyl/asparaginyl beta-hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 429 amino acids
Theoretical weight: 49.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12797 (Residues: 330-758; Coverage: 57%)
Gene names: ASPH, BAH
Sequence domains:
Structure domains: B-lactam Antibiotic, Isopenicillin N Synthase; Chain

Ligands and Environments

3 bound ligands:
Ligand MN 1 x MN
Ligand GOL 4 x GOL
Ligand OGA 1 x OGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 48.73Å b: 70.24Å c: 170.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Aspartate β-hydroxylase as a target for cancer therapy.
Kanwal et al. (2020)
3 more

2 mentions without citation

Small-molecule active pharmaceutical ingredients of approved cancer therapeutics inhibit human aspartate/asparagine-β-hydroxylase.
Brewitz et al. (2020)
1 more