› 5jzj

X-ray diffraction
1.71Å resolution

Crystal structure of DCLK1-KD in complex with AMPPN

Released:
DOI: 10.2210/pdb5jzj/pdb
PDB entry 5jzj coloured by chain, front view.
PDB entry 5jzj coloured by chain, side view.
PDB entry 5jzj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biochemical and Structural Insights into Doublecortin-like Kinase Domain 1.
Patel O, Dai W, Mentzel M, Griffin MD, Serindoux J, Gay Y, Fischer S, Sterle S, Kropp A, Burns CJ, Ernst M, Buchert M, Lucet IS
Structure 24 1550-61 (2016)
PMID: 27545623

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase DCLK1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 297 amino acids
Theoretical weight: 33.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15075 (Residues: 372-649; Coverage: 38%)
Gene names: DCAMKL1, DCDC3A, DCLK1, KIAA0369
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand SO4 1 x SO4
Ligand AN2 2 x AN2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 56.39Å b: 81.27Å c: 59.61Å
α: 90° β: 92.1° γ: 90°
R-values:
R R work R free
0.178 0.177 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

ReMAPping the microtubule landscape: How phosphorylation dictates the activities of microtubule-associated proteins.
Ramkumar et al. (2018)
2 more

7 mentions without citation

Structure-Guided Prediction of the Functional Impact of DCLK1 Mutations on Tumorigenesis.
Carli et al. (2023)
6 more