› 5jzn

X-ray diffraction
2.85Å resolution

Crystal structure of DCLK1-KD in complex with NVP-TAE684

Released:
DOI: 10.2210/pdb5jzn/pdb
PDB entry 5jzn coloured by chain, front view.
PDB entry 5jzn coloured by chain, side view.
PDB entry 5jzn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biochemical and Structural Insights into Doublecortin-like Kinase Domain 1.
Patel O, Dai W, Mentzel M, Griffin MD, Serindoux J, Gay Y, Fischer S, Sterle S, Kropp A, Burns CJ, Ernst M, Buchert M, Lucet IS
Structure 24 1550-61 (2016)
PMID: 27545623

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase DCLK1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 280 amino acids
Theoretical weight: 31.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15075 (Residues: 372-649; Coverage: 38%)
Gene names: DCAMKL1, DCDC3A, DCLK1, KIAA0369
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GUI 2 x GUI
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P61
Unit cell:
a: 106.36Å b: 106.36Å c: 157.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.181 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

ReMAPping the microtubule landscape: How phosphorylation dictates the activities of microtubule-associated proteins.
Ramkumar et al. (2018)
3 more

8 mentions without citation

Discovery of a selective inhibitor of doublecortin like kinase 1.
Ferguson et al. (2020)
7 more