› 5k0z

Electron Microscopy
2.8Å resolution

Cryo-EM structure of lactate dehydrogenase (LDH) in inhibitor-bound state

Released:
DOI: 10.2210/pdb5k0z/pdb
PDB entry 5k0z coloured by chain, front view.
PDB entry 5k0z coloured by chain, side view.
PDB entry 5k0z coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery.
Merk A, Bartesaghi A, Banerjee S, Falconieri V, Rao P, Davis MI, Pragani R, Boxer MB, Earl LA, Milne JLS, Subramaniam S
Cell 165 1698-1707 (2016)
PMID: 27238019
Related structures: EMD-8191

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase B chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 331 amino acids
Theoretical weight: 36.12 KDa
Source organism: Gallus gallus
Expression system: Bacteria
UniProt:
  • Canonical: P00337 (Residues: 2-332; Coverage: 99%)
Gene name: LDHB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-8191
Expression system: Bacteria

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Citations

118 review citations

Unravelling biological macromolecules with cryo-electron microscopy.
Fernandez-Leiro et al. (2016)
117 more

3 mentions without citation

Resolution advances in cryo-EM enable application to drug discovery.
Subramaniam et al. (2016)
2 more