› 5k53

X-ray diffraction
1.8Å resolution

Crystal structures of aldehyde deformylating oxygenase from Oscillatoria sp. KNUA011

Released:
DOI: 10.2210/pdb5k53/pdb
PDB entry 5k53 coloured by chain, front view.
PDB entry 5k53 coloured by chain, side view.
PDB entry 5k53 coloured by chain, top view.
Source organism: Oscillatoria sp.
Primary publication:
Crystal structures of aldehyde deformylating oxygenase from Limnothrix sp. KNUA012 and Oscillatoria sp. KNUA011.
Park AK, Kim IS, Jeon BW, Roh SJ, Ryu MY, Baek HR, Jo SW, Kim YS, Park H, Lee JH, Yoon HS, Kim HW
Biochem Biophys Res Commun 477 395-400 (2016)
PMID: 27329814

Function and Biology Details

Reaction catalysed: 
A long-chain aldehyde + O(2) + 2 NADPH = an alkane + formate + H(2)O + 2 NADP(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde decarbonylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 29.26 KDa
Source organism: Oscillatoria sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1D5B390 (Residues: 1-246; Coverage: 100%)
Sequence domains: Long-chain fatty aldehyde decarbonylase
Structure domains: Ferritin

Ligands and Environments

2 bound ligands:
Ligand FE 4 x FE
Ligand STE 2 x STE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 55.012Å b: 78.265Å c: 108.861Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.234
Expression system: Escherichia coli

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Citations

1 review citation

Recent advances in the improvement of cyanobacterial enzymes for bioalkane production.
Hayashi et al. (2022)
3 other articles