› 5k59

X-ray diffraction
2.84Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212095 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Uncharacterized leukocidin-like protein 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 319 amino acids
Theoretical weight: 37.14 KDa
Source organism: Staphylococcus aureus subsp. aureus USA300
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2FWP0 (Residues: 32-106, 107-337; Coverage: 94%)
Gene name: SAOUHSC_02241
Sequence domains: Leukocidin/Hemolysin toxin family
Structure domains: Leukocidin/porin MspA
Uncharacterized leukocidin-like protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 35.83 KDa
Source organism: Staphylococcus aureus subsp. aureus USA300
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2FFA3 (Residues: 30-338; Coverage: 100%)
Gene name: SAUSA300_1974
Sequence domains: Leukocidin/Hemolysin toxin family
Structure domains: Leukocidin/porin MspA
Fab heavy chain Chains: E, H
Molecule details ›
Chains: E, H
Length: 227 amino acids
Theoretical weight: 24.17 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Fab light chain Chains: F, L
Molecule details ›
Chains: F, L
Length: 214 amino acids
Theoretical weight: 23.34 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand PO4 1 x PO4
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21
Unit cell:
a: 74.79Å b: 160.932Å c: 119.484Å
α: 90° β: 101.18° γ: 90°
R-values:
R R work R free
0.199 0.197 0.237
Expression systems:
  • Escherichia coli
  • Cricetulus griseus

Quick links

Citations

12 review citations

Different drugs for bad bugs: antivirulence strategies in the age of antibiotic resistance.
Dickey et al. (2017)
11 more

8 mentions without citation

Context matters: The importance of dimerization-induced conformation of the LukGH leukocidin of Staphylococcus aureus for the generation of neutralizing antibodies.
Badarau et al. (2016)
7 more