› 5k7o

Electron crystallography
1.8Å resolution

MicroED structure of lysozyme at 1.8 A resolution

Released:
DOI: 10.2210/pdb5k7o/pdb
PDB entry 5k7o coloured by chain, front view.
PDB entry 5k7o coloured by chain, side view.
PDB entry 5k7o coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T
Nat Methods 14 399-402 (2017)
PMID: 28192420
Related structures: EMD-8217

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 1.8Å
Relevant EMDB volumes: EMD-8217

Quick links

Citations

23 review citations

MicroED in natural product and small molecule research.
Danelius et al. (2021)
22 more

9 mentions without citation

MicroED opens a new era for biological structure determination.
Nannenga et al. (2016)
8 more