› 5k7t

Electron crystallography
2.5Å resolution

MicroED structure of thermolysin at 2.5 A resolution

Released:
DOI: 10.2210/pdb5k7t/pdb
PDB entry 5k7t coloured by chain, front view.
PDB entry 5k7t coloured by chain, side view.
PDB entry 5k7t coloured by chain, top view.
Source organism: Bacillus thermoproteolyticus
Primary publication:
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T
Nat Methods 14 399-402 (2017)
PMID: 28192420
Related structures: EMD-8222

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: -|-Leu > -|-Phe.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermolysin Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.36 KDa
Source organism: Bacillus thermoproteolyticus
UniProt:
  • Canonical: P00800 (Residues: 233-548; Coverage: 61%)
Gene name: npr
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 4 x CA
Ligand ZN 1 x ZN
Ligand DMS 1 x DMS
Ligand IPA 1 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.5Å
Relevant EMDB volumes: EMD-8222

Quick links

Citations

23 review citations

Challenges and opportunities in cryo-EM single-particle analysis.
Lyumkis D. (2019)
22 more

4 mentions without citation

MicroED opens a new era for biological structure determination.
Nannenga et al. (2016)
3 more