› 5k8d

X-ray diffraction
4.19Å resolution

Crystal structure of rFVIIIFc

Released:
DOI: 10.2210/pdb5k8d/pdb
Model geometry
Fit model/data
PDB entry 5k8d coloured by chain, front view.
PDB entry 5k8d coloured by chain, side view.
PDB entry 5k8d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structural basis for the functional comparability of factor VIII and the long-acting variant recombinant factor VIII Fc fusion protein.
Leksa NC, Chiu PL, Bou-Assaf GM, Quan C, Liu Z, Goodman AB, Chambers MG, Tsutakawa SE, Hammel M, Peters RT, Walz T, Kulman JD
J Thromb Haemost 15 1167-1179 (2017)
PMID: 28397397

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132513 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Factor VIIIa heavy chain, 92 kDa isoform Chain: A
Molecule details ›
Chain: A
Length: 740 amino acids
Theoretical weight: 84.78 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P00451 (Residues: 20-759; Coverage: 32%)
Gene names: F8, F8C
Sequence domains: Multicopper oxidase
Factor VIIIa light chain; Immunoglobulin heavy constant gamma 1 Chain: B
Molecule details ›
Chain: B
Length: 865 amino acids
Theoretical weight: 99.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P00451 (Residues: 1713-2350; Coverage: 27%)
  • Canonical: P01857 (Residues: 104-328; Coverage: 56%)
Gene names: F8, F8C, IGHG1
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand CA 3 x CA
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P41212
Unit cell:
a: 136.295Å b: 136.295Å c: 365.098Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.301 0.298 0.362
Expression system: Homo sapiens

Quick links

Citations

3 review citations

Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology.
Brosey et al. (2019)
2 more

2 mentions without citation

The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
Smith et al. (2020)
1 more