› 5k8m

X-ray diffraction
2.75Å resolution

Apo 5-nitroanthranilate aminohydrolase

Released:
DOI: 10.2210/pdb5k8m/pdb
PDB entry 5k8m coloured by chain, front view.
PDB entry 5k8m coloured by chain, side view.
PDB entry 5k8m coloured by chain, top view.
Source organism: Bradyrhizobium sp.
Primary publication:
Enzymatic hydrolysis by transition-metal-dependent nucleophilic aromatic substitution.
Kalyoncu S, Heaner DP, Kurt Z, Bethel CM, Ukachukwu CU, Chakravarthy S, Spain JC, Lieberman RL
Nat Chem Biol 12 1031-1036 (2016)
PMID: 27694799

Function and Biology Details

Reaction catalysed: 
5-nitroanthranilate + H(2)O = 5-nitrosalicylate + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-nitroanthranilic acid aminohydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 425 amino acids
Theoretical weight: 46.96 KDa
Source organism: Bradyrhizobium sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: D3WZ85 (Residues: 1-425; Coverage: 100%)
Gene name: naaA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I422
Unit cell:
a: 175.27Å b: 175.27Å c: 354.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.214
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

From aniline to phenol: carbon-nitrogen bond activation via uranyl photoredox catalysis.
Hu et al. (2022)
4 more

1 mention without citation

Enzymatic hydrolysis by transition-metal-dependent nucleophilic aromatic substitution.
Kalyoncu et al. (2016)