› 5k8o

X-ray diffraction
2.89Å resolution

Mn2+/5NSA-bound 5-nitroanthranilate aminohydrolase

Released:
DOI: 10.2210/pdb5k8o/pdb
PDB entry 5k8o coloured by chain, front view.
PDB entry 5k8o coloured by chain, side view.
PDB entry 5k8o coloured by chain, top view.
Source organism: Bradyrhizobium sp.
Primary publication:
Enzymatic hydrolysis by transition-metal-dependent nucleophilic aromatic substitution.
Kalyoncu S, Heaner DP, Kurt Z, Bethel CM, Ukachukwu CU, Chakravarthy S, Spain JC, Lieberman RL
Nat Chem Biol 12 1031-1036 (2016)
PMID: 27694799

Function and Biology Details

Reaction catalysed: 
5-nitroanthranilate + H(2)O = 5-nitrosalicylate + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-nitroanthranilic acid aminohydrolase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 425 amino acids
Theoretical weight: 46.4 KDa
Source organism: Bradyrhizobium sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: D3WZ85 (Residues: 1-425; Coverage: 100%)
Gene name: naaA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 8 x MN
Ligand 6R7 8 x 6R7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 186.773Å b: 249.338Å c: 249.184Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 0.218
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

A triphenylamine-functionalized luminescent sensor for efficient p-nitroaniline detection.
Ji et al. (2018)
4 more

1 mention without citation

Enzymatic hydrolysis by transition-metal-dependent nucleophilic aromatic substitution.
Kalyoncu et al. (2016)