5kap

X-ray diffraction
2.95Å resolution

Trypanosome brucei Hypoxanthine-guanine phosphoribosyltranferase in complex with a 9-(4-(phosphonobutil)hypoxanthine

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-170435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 24.22 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07010 (Residues: 1-210; Coverage: 100%)
Gene name: HGPRT
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P2221
Unit cell:
a: 45.106Å b: 93.893Å c: 109.784Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.278 0.275 0.309
Expression system: Escherichia coli