› 5kbw

X-ray diffraction
2.61Å resolution

Crystal structure of TmRibU, the riboflavin-binding S subunit from the Thermotoga maritima ECF transporter

Released:
DOI: 10.2210/pdb5kbw/pdb
PDB entry 5kbw coloured by chain, front view.
PDB entry 5kbw coloured by chain, side view.
PDB entry 5kbw coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
An Aromatic Cap Seals the Substrate Binding Site in an ECF-Type S Subunit for Riboflavin.
Karpowich NK, Song J, Wang DN
J Mol Biol 428 3118-30 (2016)
PMID: 27312125

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194792 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin transporter RibU Chains: A, B
Molecule details ›
Chains: A, B
Length: 181 amino acids
Theoretical weight: 19.8 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X1G6 (Residues: 2-173; Coverage: 94%)
Gene names: TM_1455, ribU
Sequence domains: ECF transporter, substrate-specific component
Structure domains: Arp2/3 complex 21 kDa subunit ARPC3

Ligands and Environments

1 bound ligand:
Ligand RBF 2 x RBF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 40.142Å b: 94.365Å c: 50.986Å
α: 90° β: 95.81° γ: 90°
R-values:
R R work R free
0.206 0.202 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

ECF-Type ATP-Binding Cassette Transporters.
Rempel et al. (2019)
5 other articles

4 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
3 more