› 5kcm

X-ray diffraction
2.15Å resolution

Crystal structure of iron-sulfur cluster containing photolyase PhrB mutant I51W

Released:
DOI: 10.2210/pdb5kcm/pdb
PDB entry 5kcm coloured by chain, front view.
PDB entry 5kcm coloured by chain, side view.
PDB entry 5kcm coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Primary publication:
Crystal Structures of Bacterial (6-4) Photolyase Mutants with Impaired DNA Repair Activity.
Zhang F, Ma H, Bowatte K, Kwiatkowski D, Mittmann E, Qasem H, Krauß N, Zeng X, Ren Z, Scheerer P, Yang X, Lamparter T
Photochem Photobiol 93 304-314 (2017)
PMID: 27992645

Function and Biology Details

Reaction catalysed: 
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108864 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(6-4) photolyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 518 amino acids
Theoretical weight: 59.3 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli
UniProt:
  • Canonical: A9CH39 (Residues: 1-507; Coverage: 100%)
Gene names: Atu4765, phrB
Sequence domains: Deoxyribodipyrimidine photo-lyase-related protein
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand SF4 2 x SF4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 97.607Å b: 107.243Å c: 113.672Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.213
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Crystal-on-crystal chips for in situ serial diffraction at room temperature.
Ren et al. (2018)
5 more