› 5kd1

X-ray diffraction
1.7Å resolution

Sperm whale myoglobin H64A with nitrosoamphetamine

Released:
DOI: 10.2210/pdb5kd1/pdb
PDB entry 5kd1 coloured by chain, front view.
PDB entry 5kd1 coloured by chain, side view.
PDB entry 5kd1 coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
Nitrosoamphetamine binding to myoglobin and hemoglobin: Crystal structure of the H64A myoglobin-nitrosoamphetamine adduct.
Wang B, Powell SM, Guan Y, Xu N, Thomas LM, Richter-Addo GB
Nitric Oxide 67 26-29 (2017)
PMID: 28450187

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.17 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli
UniProt:
  • Canonical: P02185 (Residues: 2-154; Coverage: 99%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

4 bound ligands:
Ligand HEM 1 x HEM
Ligand SO4 4 x SO4
Ligand 3QM 1 x 3QM
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P6
Unit cell:
a: 90.5Å b: 90.5Å c: 45.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.148 0.177
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Insights into Nitrosoalkane Binding to Myoglobin Provided by Crystallography of Wild-Type and Distal Pocket Mutant Derivatives.
Herrera et al. (2023)
1 more

3 mentions without citation

Nitrosoamphetamine binding to myoglobin and hemoglobin: Crystal structure of the H64A myoglobin-nitrosoamphetamine adduct.
Wang et al. (2017)
2 more