› 5kd6

X-ray diffraction
1.84Å resolution

Crystal structure of the aromatic prenyltransferase AtaPT from Aspergillus terreus A8-4 in complex with dimethylallyl S-thiolodiphosphate and (-)-butyrolactone II

Released:
DOI: 10.2210/pdb5kd6/pdb
PDB entry 5kd6 coloured by chain, front view.
PDB entry 5kd6 coloured by chain, side view.
PDB entry 5kd6 coloured by chain, top view.
Source organism: Aspergillus terreus
Primary publication:
Molecular insights into the enzyme promiscuity of an aromatic prenyltransferase.
Chen R, Gao B, Liu X, Ruan F, Zhang Y, Lou J, Feng K, Wunsch C, Li SM, Dai J, Sun F
Nat Chem Biol 13 226-234 (2017)
PMID: 27992881

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103232 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aromatic prenyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 419 amino acids
Theoretical weight: 46.9 KDa
Source organism: Aspergillus terreus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1B0UHJ4 (Residues: 11-424; Coverage: 98%)
Gene name: PT
Sequence domains: Tryptophan dimethylallyltransferase

Ligands and Environments

2 bound ligands:
Ligand DST 2 x DST
Ligand LBU 1 x LBU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 96.06Å b: 134.14Å c: 68.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.228
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Protein Engineering for Improving and Diversifying Natural Product Biosynthesis.
Li et al. (2020)
10 more