› 5kdl

X-ray diffraction
2.66Å resolution

Crystal structure of the 4 alanine insertion variant of the Gi alpha1 subunit bound to GTPgammaS

Released:
DOI: 10.2210/pdb5kdl/pdb
PDB entry 5kdl coloured by chain, front view.
PDB entry 5kdl coloured by chain, side view.
PDB entry 5kdl coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
A Conserved Hydrophobic Core in Gαi1 Regulates G Protein Activation and Release from Activated Receptor.
Kaya AI, Lokits AD, Gilbert JA, Iverson TM, Meiler J, Hamm HE
J Biol Chem 291 19674-86 (2016)
PMID: 27462082

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 358 amino acids
Theoretical weight: 40.68 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10824 (Residues: 1-333, 334-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GSP 2 x GSP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 61.742Å b: 77.351Å c: 73.095Å
α: 90° β: 99.86° γ: 90°
R-values:
R R work R free
0.215 0.209 0.265
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure and dynamics of GPCR signaling complexes.
Hilger et al. (2018)
3 more

2 mentions without citation

A Conserved Hydrophobic Core in Gαi1 Regulates G Protein Activation and Release from Activated Receptor.
Kaya et al. (2016)
1 more