› 5kdx

X-ray diffraction
2.4Å resolution

IMPa metallopeptidase in complex with T-antigen

Released:
DOI: 10.2210/pdb5kdx/pdb
PDB entry 5kdx coloured by chain, front view.
PDB entry 5kdx coloured by chain, side view.
PDB entry 5kdx coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Recognition of protein-linked glycans as a determinant of peptidase activity.
Noach I, Ficko-Blean E, Pluvinage B, Stuart C, Jenkins ML, Brochu D, Buenbrazo N, Wakarchuk W, Burke JE, Gilbert M, Boraston AB
Proc Natl Acad Sci U S A 114 E679-E688 (2017)
PMID: 28096352

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Immunomodulating metalloprotease Chains: A, B
Molecule details ›
Chains: A, B
Length: 885 amino acids
Theoretical weight: 96.38 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q9I5W4 (Residues: 42-923; Coverage: 100%)
Gene names: PA0572, impA
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: A2G, GAL
Carbohydrate polymer
4 bound ligands:
Ligand PO4 5 x PO4
Ligand ZN 2 x ZN
Ligand EDO 26 x EDO
Ligand SER 2 x SER
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P21
Unit cell:
a: 99.41Å b: 133.1Å c: 103.78Å
α: 90° β: 103.12° γ: 90°
R-values:
R R work R free
0.204 0.201 0.254
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

9 review citations

Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier.
Martens et al. (2018)
8 more

7 mentions without citation

Molecular Recognition of GalNAc in Mucin-Type O-Glycosylation.
Sanz-Martinez et al. (2023)
6 more