› 5kel

Electron Microscopy
4.3Å resolution

EBOV GP in complex with variable Fab domains of IgGs c2G4 and c13C6

Released:
DOI: 10.2210/pdb5kel/pdb
PDB entry 5kel coloured by chain, front view.
PDB entry 5kel coloured by chain, side view.
PDB entry 5kel coloured by chain, top view.
Source organisms:
Primary publication:
Structures of Ebola virus GP and sGP in complex with therapeutic antibodies.
Pallesen J, Murin CD, de Val N, Cottrell CA, Hastie KM, Turner HL, Fusco ML, Flyak AI, Zeitlin L, Crowe JE, Andersen KG, Saphire EO, Ward AB
Nat Microbiol 1 16128 (2016)
PMID: 27562261
Related structures: EMD-8240

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211920 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (10 distinct):
GP1 Chains: A, E, F
Molecule details ›
Chains: A, E, F
Length: 469 amino acids
Theoretical weight: 50.97 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q05320 (Residues: 33-501; Coverage: 73%)
Gene name: GP
Sequence domains: Filovirus glycoprotein
GP2 Chains: B, G, I
Molecule details ›
Chains: B, G, I
Length: 143 amino acids
Theoretical weight: 16.14 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q05320 (Residues: 502-637; Coverage: 21%)
Gene name: GP
c2G4 variable Fab domain heavy chain Chains: H, P, Q
Molecule details ›
Chains: H, P, Q
Length: 120 amino acids
Theoretical weight: 13.57 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana
c2G4 variable Fab domain light chain Chains: L, T, U
Molecule details ›
Chains: L, T, U
Length: 107 amino acids
Theoretical weight: 11.64 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana
c13C6 variable Fab domain heavy chain Chains: C, J, M
Molecule details ›
Chains: C, J, M
Length: 121 amino acids
Theoretical weight: 13.17 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana
c13C6 variable Fab domain light chain Chains: D, N, O
Molecule details ›
Chains: D, N, O
Length: 107 amino acids
Theoretical weight: 11.61 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-8240
Expression systems:
  • Drosophila melanogaster
  • Nicotiana benthamiana

Quick links

Citations

21 review citations

The HIV-1 envelope glycoprotein structure: nailing down a moving target.
Ward et al. (2017)
20 more

13 mentions without citation

Antibodies to combat viral infections: development strategies and progress.
Pantaleo et al. (2022)
12 more