› 5kis

X-ray diffraction
2.4Å resolution

YenB/RHS2 complex

Released:
DOI: 10.2210/pdb5kis/pdb
PDB entry 5kis coloured by chain, front view.
PDB entry 5kis coloured by chain, side view.
PDB entry 5kis coloured by chain, top view.
Source organism: Yersinia entomophaga
Entry authors: Busby JN, Hurst MRH, Lott JS

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104153 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Toxin subunit YenB Chain: A
Molecule details ›
Chain: A
Length: 1491 amino acids
Theoretical weight: 167.42 KDa
Source organism: Yersinia entomophaga
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B6A880 (Residues: 1-1487; Coverage: 100%)
Gene name: yenB
Sequence domains:
RHS2 Chain: B
Molecule details ›
Chain: B
Length: 965 amino acids
Theoretical weight: 106.61 KDa
Source organism: Yersinia entomophaga
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2D0TC51 (Residues: 1-965; Coverage: 100%)
Gene name: RHS2
Sequence domains: Tripartite Tc toxins repeat
Structure domains: RHS repeat-associated core

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand NA 2 x NA
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 148.607Å b: 132.765Å c: 155.361Å
α: 90° β: 103.85° γ: 90°
R-values:
R R work R free
0.218 0.216 0.26
Expression system: Escherichia coli BL21(DE3)

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