› 5kjh

X-ray diffraction
2.27Å resolution

Crystal structure of an active polycomb repressive complex 2 in the stimulated state

Released:
DOI: 10.2210/pdb5kjh/pdb
Model geometry
Fit model/data
PDB entry 5kjh coloured by chain, front view.
PDB entry 5kjh coloured by chain, side view.
PDB entry 5kjh coloured by chain, top view.
Source organisms:
Primary publication:
Response to Comment on "Structural basis of histone H3K27 trimethylation by an active polycomb repressive complex 2".
Jiao L, Liu X
Science 354 1543 (2016)
PMID: 28008038

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-123900 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Polycomb protein EED Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 66.02 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: G0S8H7 (Residues: 1-565; Coverage: 100%)
Gene names: CTHT_0029920, EED
Sequence domains:
Histone-lysine N-methyltransferase EZH2; Polycomb protein SUZ12 Chain: B
Molecule details ›
Chain: B
Length: 937 amino acids
Theoretical weight: 106.87 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: G0SDW4 (Residues: 191-950; Coverage: 50%)
  • Canonical: G0RYC6 (Residues: 530-691; Coverage: 22%)
Gene names: CTHT_0006210, CTHT_0053230, EZH2, SUZ12
Sequence domains:
Histone H3.1 Chain: D

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand SAH 1 x SAH
1 modified residue:
Ligand M3L 1 x M3L

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID, ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 81.554Å b: 74.612Å c: 128.508Å
α: 90° β: 107.7° γ: 90°
R-values:
R R work R free
0.17 0.168 0.213
Expression systems:
  • Saccharomyces cerevisiae S288C
  • Not provided

Quick links

Citations

2 review citations

PRC2 is high maintenance.
Yu et al. (2019)
1 more

8 mentions without citation

Structure, mechanism, and regulation of polycomb-repressive complex 2.
Moritz et al. (2018)
7 more