› 5kkk

X-ray diffraction
1.7Å resolution

1.7-Angstrom In situ Mylar structure of sperm whale myoglobin (SWMb-CO) at 100 K

Released:
DOI: 10.2210/pdb5kkk/pdb
PDB entry 5kkk coloured by chain, front view.
PDB entry 5kkk coloured by chain, side view.
PDB entry 5kkk coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
A Versatile System for High-Throughput In Situ X-ray Screening and Data Collection of Soluble and Membrane-Protein Crystals.
Broecker J, Klingel V, Ou WL, Balo AR, Kissick DJ, Ogata CM, Kuo A, Ernst OP
Cryst Growth Des 16 6318-6326 (2016)
PMID: 28261000

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.37 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli
UniProt:
  • Canonical: P02185 (Residues: 1-154; Coverage: 100%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

4 bound ligands:
Ligand HEM 1 x HEM
Ligand CMO 1 x CMO
Ligand SO4 5 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P6
Unit cell:
a: 90.77Å b: 90.77Å c: 45.671Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.176 0.215
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Crystallogenesis of Membrane Proteins Mediated by Polymer-Bounded Lipid Nanodiscs.
Broecker et al. (2017)
7 more

2 mentions without citation

Discovery and Optimization of Pyrrolopyrimidine Derivatives as Selective Disruptors of the Perinucleolar Compartment, a Marker of Tumor Progression toward Metastasis.
Frankowski et al. (2022)
1 more