› 5kmu

X-ray diffraction
1.8Å resolution

CTX-M-9 beta lactamase mutant - T165W

Released:
DOI: 10.2210/pdb5kmu/pdb
PDB entry 5kmu coloured by chain, front view.
PDB entry 5kmu coloured by chain, side view.
PDB entry 5kmu coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Predicting allosteric mutants that increase activity of a major antibiotic resistance enzyme.
Latallo MJ, Cortina GA, Faham S, Nakamoto RK, Kasson PM
Chem Sci 8 6484-6492 (2017)
PMID: 28989673

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 30.97 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9L5C8 (Residues: 2-2, 4-291; Coverage: 100%)
Gene names: RCS51_P0174, bla CTX-M-9, blaCTX-M-9, blaCTX-M-9a, blaCTX-M-9b
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand PO4 4 x PO4
Ligand CL 1 x CL
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 42.743Å b: 42.212Å c: 68.035Å
α: 90° β: 104.52° γ: 90°
R-values:
R R work R free
0.161 0.159 0.184
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Conformational dynamics and enzyme evolution.
Petrović et al. (2018)
6 more

1 mention without citation

Predicting allosteric mutants that increase activity of a major antibiotic resistance enzyme.
Latallo et al. (2017)