› 5kn5

X-ray diffraction
2.8Å resolution

TGFalpha/Epiregulin complex with neutralizing antibody LY3016859

Released:
DOI: 10.2210/pdb5kn5/pdb
PDB entry 5kn5 coloured by chain, front view.
PDB entry 5kn5 coloured by chain, side view.
PDB entry 5kn5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of selectivity and neutralizing activity of a TGFα/epiregulin specific antibody.
Boyles JS, Atwell S, Druzina Z, Heuer JG, Witcher DR
Protein Sci 25 2028-2036 (2016)
PMID: 27543934

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208349 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Epiregulin Antibody LY3016859 Fab Heavy Chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 217 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Epiregulin Antibody LY3016859 Fab Light Chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 216 amino acids
Theoretical weight: 23.71 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Transforming growth factor alpha Chains: C, F
Molecule details ›
Chains: C, F
Length: 40 amino acids
Theoretical weight: 4.49 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01135 (Residues: 49-88; Coverage: 29%)
Gene name: TGFA
Structure domains: Laminin

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 63.249Å b: 164.346Å c: 64.972Å
α: 90° β: 104.21° γ: 90°
R-values:
R R work R free
0.206 0.206 0.263
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

1 review citation

Role of Epidermal Growth Factor Receptor (EGFR) and Its Ligands in Kidney Inflammation and Damage.
Rayego-Mateos et al. (2018)
3 other articles

1 mention without citation

Structural basis of selectivity and neutralizing activity of a TGFα/epiregulin specific antibody.
Boyles et al. (2016)