› 5kso

X-ray diffraction
2.25Å resolution

hMiro1 C-domain GDP-Pi Complex P3121 Crystal Form

Released:
DOI: 10.2210/pdb5kso/pdb
PDB entry 5kso coloured by chain, front view.
PDB entry 5kso coloured by chain, side view.
PDB entry 5kso coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into Parkin substrate lysine targeting from minimal Miro substrates.
Klosowiak JL, Park S, Smith KP, French ME, Focia PJ, Freymann DM, Rice SE
Sci Rep 6 33019 (2016)
PMID: 27605430

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial Rho GTPase 1 Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IXI2 (Residues: 411-592; Coverage: 29%)
Gene names: ARHT1, RHOT1
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3121
Unit cell:
a: 65.51Å b: 65.51Å c: 82.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.175 0.209
Expression system: Escherichia coli

Quick links

Citations

10 review citations

RBR ligase-mediated ubiquitin transfer: a tale with many twists and turns.
Walden et al. (2018)
9 more

2 mentions without citation

Miro1 Marks Parkinson's Disease Subset and Miro1 Reducer Rescues Neuron Loss in Parkinson's Models.
Hsieh et al. (2019)
1 more