› 5ksp

X-ray diffraction
2.16Å resolution

hMiro1 C-domain GDP Complex C2221 Crystal Form

Released:
DOI: 10.2210/pdb5ksp/pdb
PDB entry 5ksp coloured by chain, front view.
PDB entry 5ksp coloured by chain, side view.
PDB entry 5ksp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into Parkin substrate lysine targeting from minimal Miro substrates.
Klosowiak JL, Park S, Smith KP, French ME, Focia PJ, Freymann DM, Rice SE
Sci Rep 6 33019 (2016)
PMID: 27605430

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial Rho GTPase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IXI2 (Residues: 411-592; Coverage: 29%)
Gene names: ARHT1, RHOT1
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 66.07Å b: 72.64Å c: 151.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.241
Expression system: Escherichia coli

Quick links

Citations

10 review citations

RBR ligase-mediated ubiquitin transfer: a tale with many twists and turns.
Walden et al. (2018)
9 more

2 mentions without citation

Structural insights into Parkin substrate lysine targeting from minimal Miro substrates.
Klosowiak et al. (2016)
1 more