› 5ksz

X-ray diffraction
2.5Å resolution

hMiro EF hand and cGTPase domains in the GMPPCP-bound state

Released:
DOI: 10.2210/pdb5ksz/pdb
PDB entry 5ksz coloured by chain, front view.
PDB entry 5ksz coloured by chain, side view.
PDB entry 5ksz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into Parkin substrate lysine targeting from minimal Miro substrates.
Klosowiak JL, Park S, Smith KP, French ME, Focia PJ, Freymann DM, Rice SE
Sci Rep 6 33019 (2016)
PMID: 27605430

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial Rho GTPase 1 Chain: A
Molecule details ›
Chain: A
Length: 424 amino acids
Theoretical weight: 49.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IXI2 (Residues: 177-592; Coverage: 67%)
Gene names: ARHT1, RHOT1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand GCP 1 x GCP
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P43212
Unit cell:
a: 74.18Å b: 74.18Å c: 218.859Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.266
Expression system: Escherichia coli

Quick links

Citations

9 review citations

RBR ligase-mediated ubiquitin transfer: a tale with many twists and turns.
Walden et al. (2018)
8 more

6 mentions without citation

Miro: A molecular switch at the center of mitochondrial regulation.
Eberhardt et al. (2020)
5 more