› 5ktg

X-ray diffraction
2.8Å resolution

Crystal structure of mouse Bak BH3-in-groove homodimer (GFP)

Released:
DOI: 10.2210/pdb5ktg/pdb
PDB entry 5ktg coloured by chain, front view.
PDB entry 5ktg coloured by chain, side view.
PDB entry 5ktg coloured by chain, top view.
Source organisms:
Primary publication:
Assembly of Bak homodimers into higher order homooligomers in the mitochondrial apoptotic pore.
Mandal T, Shin S, Aluvila S, Chen HC, Grieve C, Choe JY, Cheng EH, Hustedt EJ, Oh KJ
Sci Rep 6 30763 (2016)
PMID: 27488021

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bcl-2 homologous antagonist/killer; Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 34.9 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-230; Coverage: 97%)
  • Canonical: O08734 (Residues: 66-144; Coverage: 38%)
Gene names: Bak, Bak1, GFP
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CR2 2 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P3121
Unit cell:
a: 171.641Å b: 171.641Å c: 98.194Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.246 0.244 0.275
Expression system: Escherichia coli

Quick links

Citations

9 review citations

BCL-2 family proteins: changing partners in the dance towards death.
Kale et al. (2018)
8 more

1 mention without citation

Assembly of Bak homodimers into higher order homooligomers in the mitochondrial apoptotic pore.
Mandal et al. (2016)