› 5ktq

X-ray diffraction
2.5Å resolution

LARGE FRAGMENT OF TAQ DNA POLYMERASE BOUND TO DCTP

Released:
DOI: 10.2210/pdb5ktq/pdb
PDB entry 5ktq coloured by chain, front view.
PDB entry 5ktq coloured by chain, side view.
PDB entry 5ktq coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Crystal structures of the Klenow fragment of Thermus aquaticus DNA polymerase I complexed with deoxyribonucleoside triphosphates.
Li Y, Kong Y, Korolev S, Waksman G
Protein Sci 7 1116-23 (1998)
PMID: 9605316

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148755 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase I, thermostable Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 61.25 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19821 (Residues: 290-832; Coverage: 65%)
Gene names: pol1, polA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DCP 1 x DCP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 109.4Å b: 137Å c: 45.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.28
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The mechanism of action of T7 DNA polymerase.
Doublié et al. (1998)
6 more

5 mentions without citation

Conformational transitions in DNA polymerase I revealed by single-molecule FRET.
Santoso et al. (2010)
4 more