› 5ku7

X-ray diffraction
2.3Å resolution

Crystal structure of the TIR domain from the Muscadinia rotundifolia disease resistance protein RPV1

Released:
DOI: 10.2210/pdb5ku7/pdb
PDB entry 5ku7 coloured by chain, front view.
PDB entry 5ku7 coloured by chain, side view.
PDB entry 5ku7 coloured by chain, top view.
Source organism: Vitis rotundifolia
Primary publication:
Structure and Function of the TIR Domain from the Grape NLR Protein RPV1.
Williams SJ, Yin L, Foley G, Casey LW, Outram MA, Ericsson DJ, Lu J, Boden M, Dry IB, Kobe B
Front Plant Sci 7 1850 (2016)
PMID: 28008335

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Disease resistance protein RPV1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 20.74 KDa
Source organism: Vitis rotundifolia
Expression system: Escherichia coli
UniProt:
  • Canonical: V9M2S5 (Residues: 20-193; Coverage: 12%)
Gene name: RPV1
Sequence domains: TIR domain
Structure domains: Toll/interleukin-1 receptor homology (TIR) domain

Ligands and Environments

1 bound ligand:
Ligand MLA 2 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Unit cell:
a: 41.855Å b: 89.117Å c: 113.858Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.18 0.235
Expression system: Escherichia coli

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Citations

5 review citations

What Do We Know About NOD-Like Receptors in Plant Immunity?
Zhang et al. (2017)
4 more

5 mentions without citation

A molecular roadmap to the plant immune system.
Bentham et al. (2020)
4 more