› 5kue

X-ray diffraction
1.5Å resolution

Human SeMet incorporated I141M/L146M mitochondrial calcium uniporter (residues 72-189) crystal structure with magnesium

Released:
DOI: 10.2210/pdb5kue/pdb
PDB entry 5kue coloured by chain, front view.
PDB entry 5kue coloured by chain, side view.
PDB entry 5kue coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Insights into Mitochondrial Calcium Uniporter Regulation by Divalent Cations.
Lee SK, Shanmughapriya S, Mok MCY, Dong Z, Tomar D, Carvalho E, Rajan S, Junop MS, Madesh M, Stathopulos PB
Cell Chem Biol 23 1157-1169 (2016)
PMID: 27569754

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185506 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium uniporter protein, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NE86 (Residues: 72-189; Coverage: 34%)
Gene names: C10orf42, CCDC109A, MCU
Sequence domains: Mitochondrial calcium uniporter

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand TRS 1 x TRS
Ligand EDO 2 x EDO
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P65
Unit cell:
a: 55.5Å b: 55.5Å c: 69.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.2 0.212
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Regulation of Calcium Homeostasis by ER Redox: A Close-Up of the ER/Mitochondria Connection.
Chernorudskiy et al. (2017)
21 more

2 mentions without citation

Mitochondrial Ca2+ transport in the endothelium: regulation by ions, redox signalling and mechanical forces.
Alevriadou et al. (2017)
1 more