› 5kum

X-ray diffraction
2.8Å resolution

Crystal Structure of Inward Rectifier Kir2.2 K62W Mutant In Complex with PIP2

Released:
DOI: 10.2210/pdb5kum/pdb
PDB entry 5kum coloured by chain, front view.
PDB entry 5kum coloured by chain, side view.
PDB entry 5kum coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structural basis of control of inward rectifier Kir2 channel gating by bulk anionic phospholipids.
Lee SJ, Ren F, Zangerl-Plessl EM, Heyman S, Stary-Weinzinger A, Yuan P, Nichols CG
J Gen Physiol 148 227-37 (2016)
PMID: 27527100

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-sensitive inward rectifier potassium channel 12 Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 39.42 KDa
Source organism: Gallus gallus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: F1NHE9 (Residues: 38-369; Coverage: 77%)
Gene name: KCNJ12
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PIO 1 x PIO
Ligand K 8 x K
Ligand DMU 1 x DMU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I4
Unit cell:
a: 81.303Å b: 81.303Å c: 183.572Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.258
Expression system: Komagataella pastoris

Quick links

Citations

6 review citations

PIP2: A critical regulator of vascular ion channels hiding in plain sight.
Harraz et al. (2020)
5 more

7 mentions without citation

Polyamines and potassium channels: A 25-year romance.
Nichols et al. (2018)
6 more