› 5kut

X-ray diffraction
1.69Å resolution

hMiro2 C-terminal GTPase domain, GDP-bound

Released:
DOI: 10.2210/pdb5kut/pdb
PDB entry 5kut coloured by chain, front view.
PDB entry 5kut coloured by chain, side view.
PDB entry 5kut coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into Parkin substrate lysine targeting from minimal Miro substrates.
Klosowiak JL, Park S, Smith KP, French ME, Focia PJ, Freymann DM, Rice SE
Sci Rep 6 33019 (2016)
PMID: 27605430

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184912 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial Rho GTPase 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 189 amino acids
Theoretical weight: 20.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IXI1 (Residues: 409-588; Coverage: 29%)
Gene names: ARHT2, C16orf39, RHOT2
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 3 x GDP
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 80.61Å b: 122.76Å c: 103.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.218 0.238
Expression system: Escherichia coli

Quick links

Citations

10 review citations

RBR ligase-mediated ubiquitin transfer: a tale with many twists and turns.
Walden et al. (2018)
9 more

2 mentions without citation

Structural insights into Parkin substrate lysine targeting from minimal Miro substrates.
Klosowiak et al. (2016)
1 more