› 5kvh

X-ray diffraction
2.27Å resolution

Crystal structure of human apoptosis-inducing factor with W196A mutation

Released:
DOI: 10.2210/pdb5kvh/pdb
PDB entry 5kvh coloured by chain, front view.
PDB entry 5kvh coloured by chain, side view.
PDB entry 5kvh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Defining NADH-Driven Allostery Regulating Apoptosis-Inducing Factor.
Brosey CA, Ho C, Long WZ, Singh S, Burnett K, Hura GL, Nix JC, Bowman GR, Ellenberger T, Tainer JA
Structure 24 2067-2079 (2016)
PMID: 27818101

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132062 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apoptosis-inducing factor 1, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 543 amino acids
Theoretical weight: 59.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95831 (Residues: 78-613; Coverage: 87%)
Gene names: AIF, AIFM1, PDCD8
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 2 x FAD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 89.845Å b: 112.166Å c: 122.211Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.24
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The MRE11-RAD50-NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair.
Syed et al. (2018)
6 more

2 mentions without citation

Defining NADH-Driven Allostery Regulating Apoptosis-Inducing Factor.
Brosey et al. (2016)
1 more